Involvement of CYP51A and CYP51B in Growth, Reproduction, Pathogenicity, and Sensitivity to Fungicides in Colletotrichum siamense

Strawberry crown rot is a serious fungal disease that poses great threat to strawberry production in the growth cycle. The dominant pathogens of were different periods. main pathogen at seedling stage unclear. In this study, 74 Colletotrichum spp. isolated from 100 plants stage. Based on morphological observations and phylogenetic analysis multiple genes (ACT, CAL, CHS, GAPDH, ITS), all tested isolates identified as C. gloeosporioides species complex, including 69 siamense 5 fructicola. Zhejiang, China. sterol demethylation inhibitors (DMIs) used control rot, their target was CYP51 gene. role homologous gene growth, reproduction, pathogenicity, sensitivity DMI fungicides has not been determined. Our study found pathogenicity CsCYP51A deletion mutants leaves stems weakened. hyphae rate CsCYP51B significantly slower than wild type, but sporulation appressorium rates increased. had increased stem. Deletion led prothioconazole, ipconazole, hexaconazole, triadimefon, prochloraz, tebuconazole, metconazole, propiconazole, difenoconazole. more insensitive. results indicate effect CsCYP51 differs.